How to differentiate a feature

This guide shows you how to compute and save the derivative of a single-cell measurement.

Prerequisite: the segmentation, tracking and measurement steps must be done for at least one population.

Reference keys: single-cell measurement

Step-by-step:

1. Open a project.

2. Set in the header the wells and positions for which you want to compute that derivative.

3. Expand the block associated with your cell population.

4. Click on the Explore table button to open the table view.

5. Go to Math > Differentiate…. If the option is disabled, check that you have cell tracks.

6. Set up the derivative computation (see celldetective.utils.derivative() for implementation details):

   • Select the measurement of interest \(m\) (e.g. area).

   • Set the window size to 1.

   • Set the derivative mode to forward.

7. Compute. A new feature d/dt.m, is written at the end of the table.

8. Go to File > Save inplace… to write this new feature in all of the position tables.