Supported File Formats

Input Formats

Celldetective supports multichannel time-lapse microscopy data saved as TIFF stacks.

Supported dimensions:

• XY (2D): Single-timepoint & single-channel image.

• CXY (3D): Single-timepoint & multichannel image.

• PXY (3D): Multi-position, single-channel & single-timepoint images.

• TXY (3D): Time-lapse images (single channel).

• PCXY (4D): Multi-position, multichannel & single-timepoint images.

• TCXY (4D): Multi-channel time-lapse images.

Supported file types:

• Standard TIFF (.tif, .tiff)

• OME-TIFF (.ome.tif, .ome.tiff)

Note

Z-stacks: Native Z-stack support is not available. However, a Z-axis can be treated as a Time-axis to segment each slice independently. There is no compatibility for data having both Z-slices and Time-points.

Metadata Handling

• Standard TIFF: Dimensions are inferred or manually configured during experiment creation.

• OME-TIFF: Axis metadata (XYZCT) is read automatically.

• Micro-Manager: Multichannel stacks acquired with Micro-Manager are deinterlaced automatically.

Tip

For large stacks (> 5 GB), use the Bio-Formats Exporter plugin in ImageJ to ensure efficient read performance.