How to annotate an event ------------------------------ This guide shows you how to annotate a single-cell event, either for manual characterisation or to create a dataset to train a deep learning event detection model. Reference keys: :term:`event`, :term:`event class`, :term:`event time` **Prerequisite:** You have accurately segmented, **tracked** and measured a cell population of interest. This guide only applies to dynamic data. Prepare the viewer ~~~~~~~~~~~~~~~~~~ #. Go to the DETECT EVENTS section for the cell population of interest. Click on the :icon:`cog-outline,black` button to set up the viewer. #. Set the modality to "grayscale". #. Set the channel of interest. #. Adapt the fraction value to your data (e.g. 0.25). At a fraction of 1, you will see the original image (but the viewer will take longer to open and may be slow). #. Set a time interval between frames (e.g. 100 ms). #. Save and exit. Annotate in the viewer ~~~~~~~~~~~~~~~~~~~~~~ #. Press the :icon:`eye-check,black` button of the DETECT EVENT section to open the event viewer. #. Adjust the image contrast. #. On the top-left side, create a new event by pressing the :icon:`plus,black` button. #. Give the event a name and set the initialization class to "no event". #. Click on a single cell to modify its event class. #. Press :icon:`redo-variant,black` **correct** on the left side to change the class. Choose a class among "event", "no event", "else" and "remove". If you pick "event", determine the exact time (in frame unit) at which the event occurs. Use the time-series of the single cell to observe inflexion points and determine precisely this time. #. Click on :icon:`redo-variant,black` **submit**, a vertical line will show you the event time on the time series. #. Proceed to annotate all single-cells with the proper class. #. Press the :icon:`export,black` button at the bottom of the left side to export a time-series dataset (in ``.npy`` format). Put the file in a folder containing all of your annotations specific to this event. #. Press the Save button to update the position table with the new event class and the values for each cell. Viewer Controls & Shortcuts ~~~~~~~~~~~~~~~~~~~~~~~~~~~ The Event Annotator provides controls to play animation, jump between frames, and quickly annotate cells using keyboard shortcuts. For a complete list of shortcuts (e.g., ``Space`` to play, ``n`` for no-event), see the :ref:`Signal Annotator Shortcuts `. Interactive Correction ~~~~~~~~~~~~~~~~~~~~~~ For efficiently refining annotations, you can use the **Interactive Plotter** to visualize and correct multiple events simultaneously based on their signal traces. #. **Open the Plotter**: While the Event Annotator is open, press ``Ctrl+P`` or select **View > Interactive Plotter** from the menu. #. **Visualize Signals**: The viewer displays the signal traces for all loaded tracks, centered on their annotated event time (t=0). You can change the displayed signal using the dropdown menu. #. **Select Tracks**: Click and drag to draw a box around traces of interest. Selected traces will be highlighted in **red**. #. **Shift Event Time**: Use the **Left** and **Right Arrow keys** to shift the event time (t0) for the selected tracks. The curves will move horizontally to reflect the new alignment. #. **Batch Reclassify**: Use the buttons at the top to assign a new class to all selected tracks: - **Event**: Confirmed event (Class 0). - **No Event**: Reject as noise or non-event (Class 1). - **Left-censored/Else**: Ambiguous or other event type (Class 2). - **Delete**: Mark for removal. #. **Save Changes**: Press **Save Changes** in the plotter to save your corrections to the disk. The main Event Annotator window will update to reflect these changes.